Literature Hub

    • Welsh JA, Goberdhan DCI, O’Driscoll L, et al; MISEV Consortium; Théry C, Witwer KW. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. J Extracell Vesicles. 2024;13(2):e12404. doi:10.1002/jev2.12404.

    • Nieuwland R, Siljander P-RM. A beginner’s guide to study extracellular vesicles in human blood plasma and serum. J Extracell Vesicles. 2024;13(1):e12400. doi:10.1002/jev2.12400.

    • Coumans FAW, Brisson AR, Buzas EI, et al. Methodological guidelines to study extracellular vesicles. Circ Res. 2017;120(10):1632-1648.

    • Dhondt B, Hendrix A, Van Deun J, et al. Benchmarking blood collection tubes and processing intervals for robust extracellular vesicle analysis. J Extracell Vesicles. 2023;12(8):e12315.

    • Karimi N, Cvjetkovic A, Jang SC, et al. Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins. Cell Mol Life Sci. 2018;75:2873-2886. doi:10.1007/s00018-018-2773-4.

    • Takov K, Yellon DM, Davidson SM. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles. 2019;8:1560809. doi:10.1080/20013078.2018.1560809.

    • Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 2014;3:23430. doi:10.3402/jev.v3.23430.

    • Vergauwen G, Tulkens J, Pinheiro C, et al. Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions. J Extracell Vesicles. 2021;10(10):e12122.

    • Puhm F, Boilard E, Machlus KR. Platelet extracellular vesicles: beyond the blood. Arterioscler Thromb Vasc Biol. 2021;41(1):87-96.

    • Welsh JA, Jones JC, Tang VA, et al. A compendium of single extracellular vesicle flow cytometry.J Extracell Vesicles. 2023;12(2):e12299.

    • Arraud N, Linares R, Tan S, et al. Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration.J Thromb Haemost. 2014;12(5):614-627.

    • Royo F, Théry C, Falcón-Pérez JM, et al. Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the International Society for Extracellular Vesicles rigor and standardization subcommittee.Cells. 2020;9(9):1955.

    • Van Deun J, Mestdagh P, Agostinis P, et al. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research. Nat Methods. 2017;14(3):228-232. doi:10.1038/nmeth.4185.

    • Welsh JA, Van Der Pol E, Arkesteijn GJA, et al. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. J Extracell Vesicles. 2020;9(1):1713526.

    • Lucien F, Gustafson D, Lenassi M, Li B, Teske JJ, Boilard E, et al. MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research. J Extracell Vesicles. 2023;12(12):e12385.

This Evidence Library is a curated, peer-reviewed reading list to support reproducible research on extracellular vesicles in blood. Articles are organized from fundamentals through pre-analytics, isolation/enrichment workflows, characterization, and common confounders (e.g., lipoproteins and platelet-derived particles). Each entry highlights what the study tested, what sample types and methods were used, and the key takeaway for experimental design. This library is selective and evolving; it is not fully representative of the entire field and is not intended to be comprehensive.