MiBlood-EV

Blood extracellular vesicles (EVs) are tiny packages released by cells that can carry signals about health and disease. But blood is also packed with other particles; like lipoproteins and abundant proteins, that can look similar to EVs and can be collected alongside them. Because no method produces perfectly “pure” EVs, the most useful goal is to clearly describe what was enriched and what else may still be present, so results can be interpreted fairly and compared across studies.

That’s where MiBlood-EV (Minimal Information for Blood EV studies) comes in. MiBlood-EV promotes clear, consistent reporting so readers can understand how samples were collected and processed, how EVs were measured, and which potential confounders were checked. This aligns with the broader push for more transparent blood EV research and sits alongside other established guidance in the field.

In practice, this means reporting key details like pre-analytical handling (timing, anticoagulant, storage), the isolation approach and settings, and core characterization readouts that support EV identity, such as particle size and concentration, imaging, marker panels (positive and negative markers), checks for common co-isolates (like lipoproteins), and other quality indicators. We encourage using the MiBlood-EV form alongside submissions so important details aren’t missed.